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ls174t human colorectal carcinoma cell line  (ATCC)


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    ATCC ls174t human colorectal carcinoma cell line
    Ls174t Human Colorectal Carcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ls174t+cells/LS+174T/pm42271423-94-3-14
    Average 97 stars, based on 1970 article reviews
    ls174t human colorectal carcinoma cell line - by Bioz Stars, 2026-09
    97/100 stars

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    Cell Culture:

    Article Title: An exopolysaccharide-rich fraction from Bifidobacterium animalis subsp. lactis MG741 ameliorates high-fat diet-induced MASLD by enhancing intestinal barrier function.
    Article Snippet: Cell viability following EPS-BL or sodium butyrate (positive control) treatment for 24 h was assessed using the CCK-8 assay (Dojindo, Tokyo, Japan). .. LS174T cells (ATCC, Manassas, VA, USA) were cultured in Advanced minimum essential medium (Advanced MEM; Gibco) supplemented with 10% FBS, 1% penicillin–streptomycin, and 1% L-glutamine at 37 ◦C in a humidified 5% CO2 atmosphere. .. For the cell viability assay, cells were seeded in 96-well plates (5 × 104 cells/well) and incubated for 24 h. After reaching confluence, cells were treated with EPS-BL (1–200 μg/ mL) or sodium butyrate (1 mM; positive control) in serum-free medium for 24 h, and viability was assessed using the Cell Counting Kit-8 (CCK-8) assay (Dojindo, Tokyo, Japan) according to the manufacturer's protocol.



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